Journal: International Journal of Molecular Sciences

Article Title: In Vitro and In Vivo Assessments of Newly Isolated N4-like Bacteriophage against ST45 K62 Capsular-Type Carbapenem-Resistant Klebsiella pneumoniae : vB_kpnP_KPYAP-1

doi: 10.3390/ijms25179595

Figure Lengend Snippet: The genome size and restriction analysis of phage KPYAP-1. The estimated size of the KPYAP-1 genome (black arrow) ( A ), and restriction patterns ( B ) were determined by pulse field gel electrophoresis. Lane M1 is the λ PFG ladder; M2 is the λ DNA digested by Hind III (New England BioLabs, MA, USA).

Article Snippet: The size was calculated by comparing the results to a standard concatenated λ PFG ladder ranging from 48.5 to 1018 kb (New England Biolabs, Ipswich, MA, USA).

Techniques: Nucleic Acid Electrophoresis

Journal: Microbial Genomics

Article Title: Chromosome-scale assembly of the streamlined picoeukaryote Picochlorum sp. SENEW3 genome reveals Rabl-like chromatin structure and potential for C4 photosynthesis

doi: 10.1099/mgen.0.001223

Figure Lengend Snippet: Pulsed-field gel electrophoresis gel slices of P. SENEW3 chromosomal DNA ( p ) against NEB Yeast Chromosome PFGE marker N0345S ( m ) at 24, 28, 32 and 36 h time points, using a Bio-Rad CHEF Mapper XA pulsed-field gel electrophoresis system.

Article Snippet: P. SENEW3 chromosomal DNA was separated using a Bio-Rad CHEF Mapper XA PFGE system.

Techniques: Pulsed-Field Gel, Electrophoresis, Marker

Journal:

Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

doi: 10.1128/JCM.41.11.4980-4985.2003

Figure Lengend Snippet: Antibiotic resistance profiles of the five major Hong Kong MRSA PFGE types at the PWH

Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

Techniques:

Journal:

Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

doi: 10.1128/JCM.41.11.4980-4985.2003

Figure Lengend Snippet: PFGE profiles of SmaI macrorestriction fragments of MRSA strains. (A) eMRSA isolates. Lanes 1, 10, and 20, λ DNA PFGE markers; lane 2, ATCC 25923; lanes 3 to 9, eMRSA-1 to -7; lanes 11 to 19, eMRSA-8 to -16. (B) Representatives of international MRSA clones and major PFGE types from the PWH. Lanes 1 and 13, λ DNA PFGE markers; lane 2, ATCC 25923; lane 3, Brazilian clone (HSJ216); lane 4, Iberian clone (HPV107): lane 5, New York/Tokyo clone (BK2464); lane 6, pediatric clone (HDE288); lanes 7 to 11, representatives of major PFGE types of MRSA from the PWH (A1, B1, C1, D, and E, respectively); lane 12, internal control strain (MRSA 3).

Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

Techniques: Clone Assay, Control

Journal:

Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

doi: 10.1128/JCM.41.11.4980-4985.2003

Figure Lengend Snippet: Correlation of phage types, FAFLP types, and PFGE types

Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

Techniques:

Journal:

Article Title: Characterization of Isolates of Methicillin-Resistant Staphylococcus aureus from Hong Kong by Phage Typing, Pulsed-Field Gel Electrophoresis, and Fluorescent Amplified-Fragment Length Polymorphism Analysis

doi: 10.1128/JCM.41.11.4980-4985.2003

Figure Lengend Snippet: Cluster analysis of the FAFLP profiles of eMRSA-1 to -16 and representatives of the international clones performed with the Dice similarity coefficient using optimization with a position band tolerance of 0.1%. The dendrogram was calculated by UPGMA. Cluster 1 (CL1) included eMRSA-2, -5, -6, -8, and -10; CL2 consisted of eMRSA-15 only; CL3 included eMRSA-12, -13, and -14; CL4 included eMRSA-1, -4, -7, -9, and -11 and the PWH isolates of PFGE types A through E; CL5 included eMRSA-3; and CL6 included eMRSA 16. Representatives of international clones also clustered separately from eMRSA isolates.

Article Snippet: A lambda DNA PFGE molecular size standard (Amersham Pharmacia Biotech, Little Chalfont, United Kingdom) and an ATCC control strain were included in each gel.

Techniques: Clone Assay